Phytochemical Screening and Lethal Toxicity of Balanite Aegyptiaca Bark (aqueous Extract) on Juvenile of Clarias Gariepinus.(burchell, 1822)

Plant with poison effect have widely been identified for a number of years, hence, there is need for the determination of a safer concentration, highest and lowest since some of this plants are used in rivers to ease catching of fish. The qualitative and quantitative analyses of the aqueous extract were conducted. This research assessed the lethal toxicity effect of aqueous extract of B. aegyptiaca on C. gariepinus juveniles. C. gariepinus were exposed to the acute concentrations of aqueous extract of the bark of B. aegyptiaca to determine the 96hLC50.The acute toxicity of water extract from dry B. aegyptiaca plant bark was conducted using the static renewal bioassay technique. The lethal concentration in which Ten (10) Juvinile fish were stocked in 50cm x 50cm x 95cm glass tank for 96hr. at concentration of 0.0g/L, 0.02g/L, 0.04g/L, 0.06g/L and 0.08g/L. The result of the experiment shows that saponin, tannins, glycoside and anthraquinones are found present in the plant extract, while steriod is absent.nd absent. The 96h-LC50 for C. gariepinus was estimated to be 0.069g/l and physiological response of the test fish was highest at 0.08g/l with lowest at 0.02g/l. The results of the study showed the toxicity effect of B. aegyptiaca on C.gariepinus juveniles. The safety level of 0.069g/l can be recommended to be applied in water body for the purpose of catching fish and also because it is biodegradable and leave no adverse effect on the environment.