Stomatal Responses to Light and Co2 in Epidermal Peels and Intact Leaves of C3 Plant of Broad Bean (vicia Faba) Species

Stomata plays a vital role in gaseous exchange therefore, understanding the signal mechanisms in response to changing environmental conditions is critically important for sustainable agriculture that will require a major reduction in plant water use due to climate change. The effect of irradiance and CO2 concentrations on mesophyll photosynthesis and stomatal conductance were conducted by physiological analysis using the novel epidermal peel experiment and infra-red gas analysis to monitor stomatal responses to dynamic environmental changes in the epidermal peel and the whole plant of Vicia faba. The approach used presented findings in the guard cell metabolism that possibly coordinate mesophyll CO2 demands with stomatal behaviour towards crop improvement. Result showed that guard cell plays a role in stomatal function even though the degree of responsiveness is slower than when the mesophyll is present. More opportunities await guard cell exploitations for better crop improvement.