Assessment of the Cell Specific Expression of the Cytochrome B6f (rieske) in the Guard Cells of Arabidopsis Thaliana (l) Plant

The role of guard cell chloroplasts and the amount and importance of guard cell photosynthesis has been of great challenge due to the complex and functional plasticity of guard cell signalling pathways. Elucidating the role of the guard cell chloroplasts and guard cell photosynthesis presents a serious challenge for researchers in the field. Electron transport in guard cells has been proposed and current research efforts towards improvement of plant productivity have also focussed on improved photosynthetic carbon assimilation and manipulation of photosynthetic enzymes. In this study, electron transport chain enzyme, the Rieske FeS protein was manipulated by molecular tools using the specific guard cell promoters and polymerase chain reaction, PCR. Transgenic Arabidopsis (Arabidopsis thaliana) plants overexpressing in the guard cells were generated showing the localization of the enzyme specifically in the chloroplast of the guard cells. This provides tools to explore importantly the potentials for manipulating stomatal behaviour towards increased crop improvement and productivity. More opportunities await the exploitation of cell specificity in the guard cells metabolism.